UV Spectrophotometric method has been developed for simultaneous estimation of dyphylline (DYP) and guaifenesin (GUA) in bulk drug and in pharmaceutical dosage form. This method utilizes distilled water as a solvent and λmax of dyphylline and guaifenesin selected for analysis was found to be 273 nm and 290 nm respectively. Linearity was observed in the concentration range of 4-12 μg/ml (DYP) (r2 = 0.9998) and 20-100 μg/ml (GUA) (r2 = 0.9997) of both drugs. The accuracy and precision were determined and found to comply with ICH guidelines. This method showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs in marketed formulation. This method was simple, rapid, accurate and sensitive.
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